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Forensic ethanol testing requires procedures that avoid introducing alcohol at the puncture site and protect evidentiary integrity. A non-alcohol antiseptic—commonly povidone-iodine—is preferred per institutional policy or kit instructions. Option B reflects this best practice. Using alcohol-based antiseptics (Options A or C) risks contaminating the specimen and can be challenged in legal proceedings, even if actual impact is minimal. Sterile water (Option D) does not adequately reduce skin flora and increases infection risk. In addition to antiseptic choice, forensic collections require strict chain-of-custody: verified identification, documented times, collector ID, kit lot numbers, tamper-evident seals, and secure transfer with signatures at each handoff. Use the specified collection tube (often gray-top NaF/KOx for glycolysis inhibition), mix gently, and package per instructions. Do not pre-label, do not discuss results, and avoid any deviation from the kit protocol. Proper antisepsis combined with meticulous documentation ensures admissibility and accuracy—key elements tested on certification exams.