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For semen analysis, motility and morphology are time- and temperature-sensitive. Patients are typically instructed to abstain from ejaculation for ~2–7 days before collection, collect by masturbation into a sterile, wide-mouth container without lubricants or condoms (unless using a lab-approved, non-spermicidal collection condom), and keep the specimen near body temperature—e.g., carried in an inner pocket—delivering it to the laboratory within about one hour. Option B captures the critical temperature and timing. Lubricants (Option A) and most condoms contain spermicidal agents or additives that impair motility. Daily ejaculation (Option C) can reduce sperm count and motility, compromising interpretation. Refrigeration (Option D) depresses motility and is inappropriate for routine analysis unless specifically directed by the lab for certain tests. Clear written instructions, documentation of collection time, and avoidance of incomplete samples (loss of initial fraction) are essential to analytic validity and are frequently assessed in non-blood specimen competencies.