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Numerous studies show that the total volume of blood cultured is the strongest determinant of pathogen recovery in bloodstream infections. For adults, each set typically requires ~8–10 mL per bottle (aerobic and anaerobic), and multiple sets from separate venipuncture sites further improve sensitivity. Option B captures this principle. Option A reduces yield; whenever possible, collect cultures before antibiotic administration. Option C ignores technique details that matter (with butterflies, inoculate aerobic first to purge air, then anaerobic). Option D is incomplete; while some patients need alternatives to iodine, best practice is chlorhexidine or povidone-iodine per policy, with adequate friction and full air-dry time; alcohol alone is less effective for skin sterilization. Additional keys include meticulous site antisepsis, disinfecting bottle stoppers with alcohol, proper order of draw (cultures first), and accurate labeling of site/time/set numbers. Emphasizing blood volume—without overfilling—directly impacts diagnostic accuracy and antimicrobial stewardship, which is why exams frequently test this concept.